Sahilah, A.M. and Son, R. and Rusul, G. and Samuel, L. and Hassan, Z. and Lum, K.Y. and Ahmad, M.A. (2000) Molecular typing of Salmonella weltevreden and Salmonella chincol by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). World Journal of Microbiology & Biotechnology, 16. pp. 621-624. ISSN 1573-0972
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Abstract
Genomic DNA of Salmonella weltevreden (10 isolates from poultry, two isolates each from raw vegetables and river water) and S. chincol (15 isolates from poultry) were characterized by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis. These isolates originated from a single location in Kajang, Selangor. The results of the PFGE and ERIC-PCR were analysed and comparisons were made using GelCompar software. ERIC-PCR with primers ERIC1R and ERIC2 discriminated the S. weltevreden into five clusters and two single isolates and S. chincol into two clusters and two single isolates at a similarity level of 80%, respectively. PFGE produced a single cluster and eight single isolates for S. weltevreden, and one cluster and 11 single isolates for S. chincol at a similarity level of 80% after digestion with the restriction enzyme XbaI, respectively. These results demonstrate that both PFGE and ERIC-PCR are suitable tools for molecular typing of the isolates examined.
Item Type: | Article |
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Uncontrolled Keywords: | ERIC-PCR, PFGE, S. chincol, S. weltevreden. |
Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
Divisions: | Academic Faculties, Institutes and Centres > Institute of Biodiversity and Environmental Conservation Faculties, Institutes, Centres > Institute of Biodiversity and Environmental Conservation |
Depositing User: | Lihan |
Date Deposited: | 24 Aug 2022 07:10 |
Last Modified: | 24 Aug 2022 07:10 |
URI: | http://ir.unimas.my/id/eprint/39294 |
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