Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7

Ooi, Wai Ling and Son, Radu and Gulam, Rusul and Mohamed Ismail, Abdul Karim and Endang, Purwati and Samuel, Lihan (2000) Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7. Pakistan Journal of Biological Sciences, 3 (1). pp. 35-37. ISSN 1812-5735

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Official URL: https://scialert.net/abstract/?doi=pjbs.2000.35.37

Abstract

A total of 30 strains of Escherichia coli O157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus (ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among the 30 E. coli O157:H7 strains. Nearly every isolates had a unique fingerprint and that there were no bands that were highly conserved among the isolates. This study suggests that there is considerable genetic heterogeneity among the E. coli O157:H7 strains by ERIC PCR, and that this has application in screening strains from clinical or food samples to detect a virulent strain with a known fingerprint, and to trace its dissemination.

Item Type: Article
Uncontrolled Keywords: Escherichra coli O157:H7, ERIC-PCR.
Subjects: Q Science > Q Science (General)
Divisions: Academic Faculties, Institutes and Centres > Institute of Biodiversity and Environmental Conservation
Faculties, Institutes, Centres > Institute of Biodiversity and Environmental Conservation
Depositing User: Lihan
Date Deposited: 18 Aug 2022 03:20
Last Modified: 18 Aug 2022 03:20
URI: http://ir.unimas.my/id/eprint/39249

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