Son, Radu and Ooi, Wai Ling and Siripen, Srimontree and Aroonluk, Lulitanond and Wong, Foo Hin and Yuherman, . and Samuel, Lihan and Gulam, Rusul and Abdul Rahim, Mutalib (2000) Characterization of Burkholderia pseudomallei isolated in Thailand and Malaysia. Diagnostic Microbiology and Infectious Disease, 38 (3). pp. 141-145.
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Abstract
A total of 35 Burkholderia pseudomallei isolates from Thailand (16 clinical and eight soil isolates) and Malaysia (seven animal, two isolate each from clinical and soil) were investigated by their antimicrobial resistance, plasmid profiles and were typed by randomly amplified polymorphic DNA analysis. All isolates were found to be resistant to six or more of the 12 antimicrobial agents tested. Only two small plasmids of 1.8 and 2.4 megadalton were detected in two clinical isolates from Thailand. RAPD analysis with primer GEN2-60-09 resulted in the identification of 35 RAPD-types among the 35 isolates. The constructed dendrogram differentiated the 35 isolates into two main clusters and a single isolate. The wide genetic biodiversity among the 35 isolates indicate that RAPD-PCR can be a useful method to differentiate unrelated B. pseudomallei in epidemiological investigation.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Academic Faculties, Institutes and Centres > Institute of Biodiversity and Environmental Conservation Faculties, Institutes, Centres > Institute of Biodiversity and Environmental Conservation |
| Depositing User: | Lihan |
| Date Deposited: | 18 Aug 2022 02:56 |
| Last Modified: | 18 Aug 2022 02:56 |
| URI: | http://ir.unimas.my/id/eprint/39248 |
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