Improvement of extraction and stability of anthocyanins, the natural red pigment from roselle calyces using supercritical carbon dioxide extraction

Zuhaili, Idham and Nicky Rahmana, Putra and Ahmad Hazim, Abdul Aziz and Ahmad Syahmi, Zaini and Noor Azwani, Mohd Rasidek and Norlisa, Mili and Mohd Azizi, Che Yunus (2022) Improvement of extraction and stability of anthocyanins, the natural red pigment from roselle calyces using supercritical carbon dioxide extraction. Journal of CO2 Utilization, 56 (101839). pp. 1-11. ISSN 2212-9820

PDF anthocyanins1.pdf Download (251kB)

Abstract

In this work, anthocyanins, a natural red pigment, were isolated from roselle calyces using supercritical carbon dioxide (SC-CO2). Three process conditions, namely pressure, temperature, and co-solvent ratio (ethanol-water), were investigated using a three-factorial design from response surface methodology (RSM). The method was used to model the extraction of anthocyanins, total phenolic content, total flavonoid content, and colour characteristics (i.e., lightness (L*), chroma (C*), and hue (h◦)). The best conditions were 27MPa, 58 ◦C, and 8.86 % cosolvent ratio at maximal anthocyanin, phenolic, and flavonoid content, with minimal L* and C* values. The anthocyanin production was 1197 mg/100 g of dried roselle calyces. Next, the high relative value of cyanidin 3- sambubioside and phenolic compound in SC-CO2 extract was analyzed using an ultra-high-performance liquid chromatogram (UHPLC). Anthocyanins stored at 4 ◦C, 25 ◦C, and 37 ◦C had average reaction rate (k) and half-life (t1/2) values of 0.0032 and 216 days, 0.0098 and 70 days, and 0.024 and 28 days for SC-CO2 and 0.0093 and 74 days, 0.0222 and 31 days, and 0.0444 and 15 days for solid-liquid extraction (SLE), respectively, in first-order degradation kinetics. These findings demonstrated that the studied conditions using SC-CO2 provided lower degradation rates and a longer t1/2 than the conventional SLE methods

Actions (For repository members only: login required)

View Item