Isolation And Identificaton Of Xylose Utilizing Yeasts From Fruit Surfaces

Nurhayati, Mawi (2019) Isolation And Identificaton Of Xylose Utilizing Yeasts From Fruit Surfaces. [Final Year Project Report] (Unpublished)

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Abstract

The conversion of pentoses into ethanol have become a challange and could increase the demand of supply of second-generation biofuels. This study were attempted to isolate naturally occuring yeasts from different kind of local fruits in Malaysia at different places and determine the yeasts strain that can utilize xylose. Isolation processes were conducted with medium that containing only xylose as the carbon source. Four yeast strains with the ability to utilize xylose were obtained from papaya in Matang, papaya in Samarahan, longan in Samarahan and banana in Matang. The strains selected were characterized by morphological and they were identified by homology analysis of 26-S ribosomal DNA gene sequences. The identities of four yeast strains are identified as Debaryomyces nepalensis, Geotrichum candidum, Trichsoporon asahii and Candida tropicalis. All of these strains can utilize xylose as carbon source to obtain ethanol production except Debaryomyces nepalensis which produce xylitol as major metabolites.

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