Ex vitro acclimatization of tissue cultured MD2 Pineapple

Lesley, Juilih (2018) Ex vitro acclimatization of tissue cultured MD2 Pineapple. [Final Year Project Report] (Unpublished)

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Abstract

MD2 Pineapple (Ananas comosus) is one of a commercially grown variety of pineapple categorized under the family Bromeliaceae (sub-family: Bromelioideae). It is favoured compared to other varieties due to the good qualities such as physical appearance, texture, taste, and others. Due to these qualities, the market demand for MD2 Pineapple is increasing over time. However, the conventional method of planting is not able to fulfil the market demand. As an alternative, tissue culture is used to open opportunities to increase the production of MD2 Pineapple. Thus, this experiment was conducted to improve the acclimatization step, which is one of the limiting factors towards the success of tissue cultured plants. The in vitro cultured plantlets were tested in a different environment which is left outside lab (control), inside the bottle and, using mini-greenhouse for 5 weeks of acclimatization period to find out if there is a significant difference in each environment. The result shows there is a significant difference for plantlets inside the mini-greenhouse compared to the plantlets left outside lab (control) and inside the bottle. However, there is no significant difference between plantlets covered with bottle compared with the control. So, it can be concluded that the mini-greenhouse is the most suitable environment for the plantlets to be acclimatized.

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