Heterocyclic Hydrocarbon Degradation using brio diabolicus Strain MRT

Nur Izza Diyana, Ismail (2018) Heterocyclic Hydrocarbon Degradation using brio diabolicus Strain MRT. [Final Year Project Report] (Unpublished)

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Abstract

Exposure to high amount of hydrocarbons contamination caused hazardous effects to living organisms and cause major environmental problem. Fortunately, bacteria able to degrade the heterocyclic hydrocarbons even toxic. Biodegradation and bioremediation are techniques that are preferred to solve this problem because they have many advantages. Hence, this study was conducted to measure the degradation efficiency of heterocyclic hydrocarbon carbazole (CAR), dibenzothiophene (DBT) and dibenzofuran (DBF) by Vibrio diabolicus isolated from mangrove environment using gas chromatography-flame ionization detector (GC-FID) and to observe the bacterial growth on polycyclic aromatic hydrocarbon. Growth of bacteria was done through enrichment process using liquid enrichment media and through streaking onto solid media to obtain pure culture and ensure growth process. Enumeration of bacteria and optical density of bacteria were done to confirm the growth of bacteria. The bacterial growth on Mineral Salt Media (MSM) supplemented with 0.1% (v/v) CAR, DBT and DBF were determined by using spread plate method and optical density value at 600 nm. The enumeration of bacteria was calculated to determine the amount of viable colony in 0.1mL bacteria culture. The bacteria growth increases after being incubated for 14 days. Strain MRT were able to grow on MSM agar with concentrations from 0.3% (v/v) up to 1.2% (v/v) substrates. The bacteria showed the highest degradation with CAR at 90.41% followed by DBT at 63.21% and DBF at 58.49%. Strain MRT was also able to grow on MSM agar supplemented with 0.1% (v/v) fluorene, phenanthrene, pyrene and naphthalene. Based on these results, V. diabolicus were able to degrade heterocyclic hydrocarbon and PARs as their sole carbon source.

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