Ex vitro acclimatization of tissue cultured Borneo's endemic orchid, Vanda dearei

Lee, Tian Wee (2018) Ex vitro acclimatization of tissue cultured Borneo's endemic orchid, Vanda dearei. [Final Year Project Report] (Unpublished)

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Abstract

Vanda dearei belongs to Orchidaceae family which is innate to the Borneo Island. It is well-known due to large, pale yellow and strong fragrant flowers. Hybridists choose V. dearei in breeding of the hybrid flowers due to the yellow breeding lines and spontaneous flowering. Modem tissue culture methods such as in vitro germination and micropropagation help to produce vast amount of plant products including Vanda dearei. However, acclimatization stage is an obstruction of micropopagation technique due to vast amount of plants die. This study was aimed to establish the suitable treatments for acclimatization of Vanda dearei. The plantlets were subjected to different acclimatization approaches. There is a control and two treatments [treatment 1 (charcoal and coconut fibre) and treatment 2 (charcoal and peat moss)]. The results show that there was significant higher in percentage of plant survival rate, plant height, number of roots and leaves in treatment 2 compared to control and treatment 1. Hence, it can be concluded that treatment 2 (charcoal and peat moss) is the suitable treatment to acclimatize Vanda dearei.

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