Antibiotic Susceptibility and Detection of Antibiotic Resistant Genes In Intermediate Leptospira

Chong, Wan Xuan (2018) Antibiotic Susceptibility and Detection of Antibiotic Resistant Genes In Intermediate Leptospira. [Final Year Project Report] (Unpublished)

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Abstract

Leptospira are thin, highly motile, Gram negative obligate aerobic bacteria, responsible in causing leptospirosis. Phylogenetically, they are grouped into three clusters: saprophytic, intermediate and pathogenic. Intermediate Leptospira is able to cause disease in human and animals though it is less frequent. The global issue of antibiotic resistance has been rapidly emerging due to the occurrence of antibiotic-resistant pathogens. The emergence of antibiotic-resistant Leptospira strains cannot be ruled out due to the overuse of antibiotics, and the identification of genes with a potential to be involved in antibiotic resistance is critical. The main objective of this study is to detect antibiotic resistant genes, blaTEM--1 and tet(B), in selected intermediate Leptospira strains. Initially, antibiotic susceptibility testing was performed using broth microdilution method, followed by DNA extraction. Polymerase Chain Reaction was then conducted using specific primers to amplify the targeted genes, followed by agarose gel electrophoresis. From this study, the results of antibiotic susceptibility testing were analyzed based on the minimum inhibitory concentration (MIC), in which ampicillin and streptomycin had the highest MIC values (800 to >1600 tg/mL) compared to the other antibiotics. However, both antibiotic resistant genes were not detected in the selected intermediate Leptospira isolates, indicating an absence of the two antibiotic resistant genes in the respective isolates. The results from this study suggest that antibiotic resistance is not conferred by b/aTEM-1 and tet(B) genes.

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