Heterocyclic Hydrocarbon Degradation using Idiomarina sp. strain ST2

Ainul Syukrina, Mohamad Zam Zam (2018) Heterocyclic Hydrocarbon Degradation using Idiomarina sp. strain ST2. [Final Year Project Report] (Unpublished)

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Abstract

Heterocyclic hydrocarbons are major components of oil. Oils spills in sea water has cause concern as it is harmful to fish, shellfish, mammals, marine birds and human when exposed to them. This study was conducted to measure the degradation efficiency of heterocyclic hydrocarbon carbazole (CAR), dibenzothiophene (DBT) and dibenzofuran (DBF) by bacteria Idiomarina sp. ST2 isolated from seawater using gas chromatography-flame ionization detector (GC-FID) analysis and to observe the bacterial growth on polycyclic aromatic hydrocarbons (PAHs). The bacterial growth on ONR7a medium supplemented with 0.1 % v/v CAR, DBT and DBF were determined by using spread plate method and optical density value at 600nm wavelength. The bacteria growth increased after being incubated for 15 days. The bacteria showed the highest CAR degradation at 64.17 % followed by DBF (48.94 %) and DBT (31.5 %) after 14 days. Additionally, strain ST2 were able to grow on ONR7a agar supplemented with 0.1 % v/v naphthalene, phenanthrene, pyrene and fluorene, a polycyclie aromatic hydrocarbons. Based on these results, Idiomarina sp. ST2 were able to degrade heterocyclic hydrocarbon and PAHs as their sole carbon source.

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