McEwing, R. and Ouitavon, K. and Rovie-Ryan, J. J. and M., Wulansari and Sitam, F. T. and Ogden, R. (2011) Molecular sexing of tigers, Panthera tigris. Conservation Genetics Resources, 4 (2012). pp. 299-301. ISSN 1877-7252
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Abstract
We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. The ZFX and ZFY amplicons differ in size by 12 bp and can thus be differentiated by capillary electrophoresis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Molecular sex identification Tiger Zinc finger protein Forensics Felids, UNIMAS, University, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, Universiti Malaysia Sarawak |
| Subjects: | Q Science > QL Zoology |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology |
| Depositing User: | Ryan Japning |
| Date Deposited: | 06 Sep 2021 00:57 |
| Last Modified: | 06 Sep 2021 00:57 |
| URI: | http://ir.unimas.my/id/eprint/36007 |
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