Isolation And Characterization Of Thermophilic Bacteria From Annah Rais And Panchor Hot Springs

Ng, Wing Woh (2018) Isolation And Characterization Of Thermophilic Bacteria From Annah Rais And Panchor Hot Springs. [Final Year Project Report] (Unpublished)

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Abstract

Thermophilic bacteria are one of the most utilized bacteria in industrial sector due to their ability to withstand high temperature. Hence, by knowing the characteristic of thermophilic bacteria can boost the advancement of industries and also clinical researches. Annah Rais and Panchor hot springs are 2 known hot springs in Sarawak, Malaysia which are believed to have high numbers of thermophilic bacteria and may present some unknown species yet to be discovered. The' aim of this research is to isolate and identify thermophilic bacteria in the mentioned hot springs and to screen for their potential industrial application. The methods of this research were based on the isolation and characterization of thermophilic bacteria in Annah Rais and Panchor hot springs. DNA sequences were used to identify the strains by using 16S rRNA sequencing and DNA fingerprinting. Seven isolates were successfully isolated from the hot springs and identified. Besides, they were tested with several biochemical and enzymatic production tests. In conclusion, thermophilic bacteria isolated from Annah Rais and Panchor hot springs can be further investigated for potential industrial application

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