Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM

Bay, Hui Han (2008) Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM. Masters thesis, Universiti Malaysia Sarawak (UNIMAS).

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Abstract

The axenic seedlings were kept in vitro as the source axenic explants. Shoot-tip explants derived from axenic off shoots were cultured in Gamborg B5 media incorporated with 30% sucrose, 3 g/L gelrite and different concentrations of cytokinins i.e. TDZ, BAP alone or in combination with NAA in the direct regeneration pathway. The primers produced amplification products which were monomorphic across the mother plants and the micropropagated plantlets, confirming the reliability of developed micropropagation protocol to produce true-to-tpe plantlets. Both leaves and leaf sheaths were used for callus induction in the indirect regeneration pathway.

Item Type: Thesis (Masters)
Additional Information: Thesis (Master of Science) -- Universiti Malaysia Sarawak, 2008.
Uncontrolled Keywords: genetic, Zingiberaceae, ginger, Universiti Malaysia Sarawak, UNIMAS, IPTA, education, universiti, university, kuching, sarawak, malaysia, samarahan, Postgraduate, research
Subjects: Q Science > QK Botany
Divisions: Academic Faculties, Institutes and Centres > Institute of Biodiversity and Environmental Conservation
Faculties, Institutes, Centres > Institute of Biodiversity and Environmental Conservation
Depositing User: Karen Kornalius
Date Deposited: 03 Jul 2014 05:51
Last Modified: 13 Apr 2023 03:59
URI: http://ir.unimas.my/id/eprint/3588

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