Protein profiling of mutant type and wild type of Leucaena leucocephala

Amirah Fasya, Jama (2018) Protein profiling of mutant type and wild type of Leucaena leucocephala. [Final Year Project Report] (Unpublished)

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Abstract

Leucaena leucocephala is also known as Petai Belalang tree. This leaf of L. leucocephala has many benefits toward human and animals such as fodder, fuel and charcoal. The proteins were extracted by using the three different methods; phenol, TCA and imidazole for mutant and wild type of L. leucocephala. The extraction methods have different substances in the extraction buffer that can give the process of extraction become more efficient. The purpose of this study is to identify the most suitable methods for extraction the proteins between phenol, TCA and imidazole methods by observing the band intensity on the gel of SDS-PAGE. TCA method has shown the clear and bright bands for wild type and mutant of L. leucocephala. Therefore, the TCA is the most suitable method method among phenol and imidazole has proceeded into 2-D PAGE whereby there is difference in spot intensity for wild type is 0.557 while mutant is 0.363 by using Delta2D software.

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