Screening of local herbs against Asperfillus flavus

Shubashini, Baktha Vachil (2018) Screening of local herbs against Asperfillus flavus. [Final Year Project Report] (Unpublished)

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Abstract

This study focused on screening of local herbs against Aspergillus flavus. Three plant extracts were used in this study. They were Carica papaya (papaya), Piper belle (betel) and Hibiscus rosasinensis (hibiscus) leaves. Methanol extracts leaves of these three plants was screened for their antifungal activity against A. flavus. For antifungal assay, poisoned food technique and disc diffusion before or after growth of A. flavus was applied. Different concentrations (50, 75 and 100 mg/ml) were prepared from the plant extracts. C. papaya leaf showed the highest percentage of inhibition of fungi growth at 100 mg/ml of methanol leaf extract followed by 75 and 100 mg/ml of P. belle leaf and 50mg/ml of H. rosasinensis leaf extract. In disc diffusion before the growth of A. flavus, the zone of inhibition was not formed in all concentrations of the methanol leaf extracts. For disc diffusion after growth of A. flavus there was partial zone of inhibition at I00mg/m1 of C. papaya leaf, 75 and 100 mg/ml for P. belle leaf and for H. rosasinensis leaf was at 50 mg/ml of leaf extract. Using this poisoned food technique, the different concentrations of methanol leaf extracts have shown inhibition against A. flavus and for disc diffusion after growth of A. flavus have shown partial zones of inhibition. The highest percentage of inhibition for antifungal activity was observed in poisoned food technique.

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