Assessment Of The Effectiveness Of Chlorine Solution As Sterilization Method On Burkholderia Pseudomallei And Burkholderia Species In Laboratory

Angeline, Michael (2018) Assessment Of The Effectiveness Of Chlorine Solution As Sterilization Method On Burkholderia Pseudomallei And Burkholderia Species In Laboratory. [Final Year Project Report] (Unpublished)

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Abstract

In this study, the method of sterilizing Burkholderia pseudomallei and Burkholderia species were using chlorine solution, which is known as one of the cheapest disinfectant for wide scope of microorganisms. The effectiveness of chlorine sterilization was evaluated at different concentrations of chlorine with variating contact time and shelf-lives of the chlorine solution. Results indicated that chlorine concentration is effective in the sterilization of Burkholderia species and Burkholder ia pseudomallei in the range of at least 15 mg/L. In addition, the effectiveness of the chlorine solution is maintained if used within 2 days of preparation with no significant effect out from the aspect of the contact time. Hence, effectiveness of chlorine solution to sterilize B. pseudomallei and Burkholderia species were in chlorine concentration of 15 mg/L made within 48 hours from preparation date.

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