Isolation and Sequence Analysis of CLE (CLAVATA3/Endosperm Surrounding Region-related) Gene in White Kelampayan (Neolamarckia cadamba)

Tee, Alaythia Zhen Shi (2018) Isolation and Sequence Analysis of CLE (CLAVATA3/Endosperm Surrounding Region-related) Gene in White Kelampayan (Neolamarckia cadamba). [Final Year Project Report] (Unpublished)

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Abstract

Neolamarckia cadamba (kelampayan) is an economically important tree in Malaysia as its demand in many industries are high due to its fast-growing property. In establishing tree improvement programme and management, numerous researchers had conducted studies on the contributing factors of the mechanism of wood fonnation ofkelampayan. In many plants, CLE genes are found to be crucial players in cell-to-cell communication that contributes to secondary plant growth and development. Few short and incomplete CLE genes available in the genomic database of kelampayan brought forth the need of studying and amplifying the partial genomic sequence of CLE genes in kelampayan. Several parameters of molecular techniques such as DNA extraction, PCR and AGE were used in perfonning this study. However, the PCR product sent for sequence analysis was found to be smearing". The reason for DNA smearing was carry-over contamination. Thus, the partial CLE gene sequence was not available for sequencing.

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