Fed batch Lactic Acid Fermentation Using Enterococcus Faecalis

Nur Saidatul Izuanis, Salleh (2018) Fed batch Lactic Acid Fermentation Using Enterococcus Faecalis. [Final Year Project Report] (Unpublished)

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Abstract

Enterococcus faecalis strain was used to perform lactic acid production from hydrolysate sago starch. E. faaecalis is one of the lactic acid bacteria that is capable of producing LA via fermentative route. The demand of the LA is increasing over the year as the uses of the LA has been recognized and accepted over the world. Hence, this recognition has put the LA in high demand and interest in its production as well as in research studies. In this study, the fermentation conditions was kept at 37°C and pH of 7.0. Strategy used in this study is by using fed batch feeding system. It was found that these parameters were conducive for the growth of the strain and producing LA. According to the results of the experiment, the yield of LA produced by E.faecalis in fed batch mode has increased 22.68% of LA production significantly. Fed batch lactic acid fermentation has recorded about 0.741 g/Lh as compare to batch lactic acid fermentation which recorded only 0.604 g/Lh. Lactic acid produced by E.faecalis is 93.50 g/L in 112 hours of incubation period from 120g of glucose used. Throughout this experiment, about 13.54% ofNaOH was used in neutralizing the system during the production of LA.

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