Potential of Bioethanol Waste Stream as a Feedstock for Heterologous Expression of Amylase in Recombinant Escherichia coli BL21

Affiqa Nadzwa, Mohd Shafeeq Abdullah (2018) Potential of Bioethanol Waste Stream as a Feedstock for Heterologous Expression of Amylase in Recombinant Escherichia coli BL21. [Final Year Project Report] (Unpublished)

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Abstract

Bioethanol fermentation by Saccharomyces cerevisiae produces several by-products including glycerol, which potentially can be exploited as a carbon source for microbial fermentations. The low cost of the bioethanol waste stream, if it is to be used as a fermentation feedstock, offers an attractive economic benefit in the production ofrecombinant proteins. This project aims to investigate the feasibility of bioethanol waste stream as a feedstock for microbial fermentations. Specifically, this work will be focusing on the heterologous expression of amylase in Escherichia coli BL2 l. Throughout this work, the influence of temperature on the expression of the abovementioned enzymes was studied. The results of this project showed that the waste stream arising from bioethanol fermentation was feasible to be used as a fermentation feedstock for production of heterologous amylase in recombinant E. coli BL21. It was also shown that conducting the fermentations at 37°C during pre-induction and 30°C during post-induction resulted in high amylase activity (1.83 U/mL) compared to that at 37°C (1.72 U/mL) and 30°C (1.28 U/mL).

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