Isolation and Characterization of Shigella spp. from Water in Aquaculture Farm

Stephanie Lenja, Sait (2018) Isolation and Characterization of Shigella spp. from Water in Aquaculture Farm. [Final Year Project Report] (Unpublished)

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Abstract

Shigellosis, the clinical presentation for Shigella spp. infection is characterized by abdominal cramps, fever, and diarrhea included four groups S.sonnei, S.boydii, S.dysentery and S.flexneri, Therefore, Shigella are responsible pathogen for majority of bacillary dysentery cases. However, their presences in aquaculture sector had become a concern, especially affect towards to human health. Besides, treatment of shigellosis with appropriate antimicrobial agents is able to shorten duration of illness the consequences are they able to resists against antimicrobial agents are increasing. The general aim for this study was to isolate and identification the presences of Shigella spp. in both traditional and commercial aquaculture farm. In order to achieve this, water sampling collection from I traditional pond and 2 aquaculture pond, isolated by XLD agar, differentiated by several biochemical tests and molecular analysis (polymerase chain reaction). Amplification of Shiga toxin (stx gene) was done to confirm the presences of pathogenic Shigella spp. in both type farms. Three cultures samples from each aquaculture farm were assessed with some appropriate antibiotics such as ampicillin, tetracycline, sulphonamides, erythromycin and sulfamethoxazole. Overall, the bacterial strain are able to resist the antibiotics.

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