Isolation and Characterization of Vibrio cholerae from Water in Aquaculture Farms

Laurie, Ali (2018) Isolation and Characterization of Vibrio cholerae from Water in Aquaculture Farms. [Final Year Project Report] (Unpublished)

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Abstract

Aquaculture is an important sector in agriculture that supply protein source. However, the presence of pathogenic bacteria in aquaculture products may cause serious health problems. Vibrio cholerae is one of pathogenic bacteria that can cause food borne disease. In this study, we aim to isolate Vibrio cholerae isolates from water in commercial and traditional farms. Isolation was done using selective growth on TCBS agar, morphological and three biochemical identifications. A total of 12 Vibrio cholerae isolates were confirmed out of a total of 60 presumptive isolates. The isolates were later further confirmed using specific PCR targeting ctxA gene to detect pathogenic strain. ctxA encodes for cholera toxin gene. All 12 isolates do not harbor ctxA genes. Antibiotic susceptibility test was also done on the 12 isolates and recorded a high antibiotic resistance profile. This study revealed the antibiotic resistance pattern of Vibrio cholerae towards antimicrobial agents. Constant monitoring on antibiotic resistance pattern of Vibrio cholerae in aquaculture farms should be done to prevent future resistance problems.

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