Identification of protein partners of the human ribosomal protein, eS31 using Bioinformatic Analysis

Leong, Kar Xin (2018) Identification of protein partners of the human ribosomal protein, eS31 using Bioinformatic Analysis. [Final Year Project Report] (Unpublished)

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Abstract

Ribosomal protein(RPs) are one of the most important component found in ribosome that play an essential roles in ribosome biogenesis and includes some extraribosomal functions. Apart from these functions, RPs also often associated with cancer and disease development. Previous study indicated that there is low expression of eS3 l in NPC cells is unrelated to p53 expression pattern. However, the protein partners and its function resulting in this tumor-suppressing properties and the function of other protein-protein interacting partners still remains unclear. An approach known as Bioinfonnatic analysis is used to derive the possible protein interacting partners based on computational approach data analysis. This method can be divided into 4 basic steps, which are identification of target and template sequences, three-dimensional protein modelling, identification of structural neighbours and candidate partners and molecular docking analysis. By using this approach, eS3 l 3D structural model has constructed that have allowed the prediction of 11 O structural neighbors. From these, there are 5 of them which are from human origin were selected for further analysis based on logical prospect of l;iinary protein-protein interactions. Further analysis revealed that there are 3 candidate docking partners. Thus, this research has provided theoretical evidence for the interactions between eS3 l and other protein partners. Then, it has been predicted that there are 3 potential protein partners that might play distinct roles and functions from its interaction with eS3 I.

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