Evaluation of antimicrobial activity of leaf extract of Canarium Eodontophyllum Miq (Dabai) in Sarawak

Ainna Ezzaty, Yusof (2018) Evaluation of antimicrobial activity of leaf extract of Canarium Eodontophyllum Miq (Dabai) in Sarawak. [Final Year Project Report] (Unpublished)

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Abstract

Canarium odontophyllum which is also known as 'Dabai' is one of the popular underutilized fruits in Sarawak. This study was conducted to extract the leaf using methanol, ethanol and acetone as solvent by maceration method with the evaluation of antimicrobial activity using agar well diffusion method. The concentrations of the extracts used are 100 mg/mL, 50 mg/mL, 25 mg/mL and 12.5 mg/mL. The leaf extracts were screened against two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and two Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) using different solvent extracts. The findings showed that only gram positive bacteria (s.aureus and B.cereus) was found to be susceptible towards the leaf extracts of methanol, ethanol and acetone compared to gram negative bacteria. Escherichia coli and Pseudomonas aeruginosa were resistant towards the methanol, ethanol and acetone extracts. These findings will be useful for future study on using C.odontophyllum leaves as a new drug in the treatment of bacterial infections.

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