Cytogenetic Analysis Of Rasbora Sarawakensis

Hii, Deborah Siew Hie (2018) Cytogenetic Analysis Of Rasbora Sarawakensis. [Final Year Project Report] (Unpublished)

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Abstract

Rasbora sarawakensis is a species of ray-finned fish which belongs to the genus Rasbora. Currently, Rasbora establishes the most species-rich genus in the cyprinid subfamily Danioninae with 77 species. However, the characterization and chromosomal configuration of R. sarawakensis species still remains limited due to cryptic species diversity. Hence, in order to clarify unresolved issues of R. sarawakensis characterization and chromosomal configuration, cytogenetics analysis had been conducted on this species. In this research, R sarawakensis was soaked aerobically in 0.01 % colchicine solution for 1 O hours in order to inhibit the spindle fibres formation, thus arresting the cells in the metaphase stage. In the preparation of chromosome, eight different organs were isolated, followed by sufficient sterilization in order to avoid any contamination. Once the cells were arrested, fixation was performed using absolute ethanol and glacial acetic acid in the ratio of 3: 1. The cells were dropped on several slides and were then stained with Giemsa stain to study the banding pattern on the chromosome. However, in this study, the chromosome obtained after giemsa staining appeared to be deteriorated and broken in their sister chromatids, hence only abnormal chromosomes were observed. Further studies on karyotyping method are needed to obtained better and clearer result.

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