Vallirie Easter, Johnson (2018) Antimicrobial Activity of Red Jerangau (Boesenvergia stenophylla) Extract Against Staphylococcus aureus, Salmonella typhimurium, and Listeria monocytogenes. [Final Year Project Report] (Unpublished)
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Abstract
Red jerangau (Boesenbergia stenophylla), found mainly in the Borneo highland, is a rare species of ginger used in folklore remedies to treat food poisoning, alcohol intoxication, and stomach aches. In this study, extracts of B. stenophylla were tested for antimicrobial properties against Staphylococcus aureus, Salmonella typhimurium, and Listeria monocytogenes, all of which may cause food poisoning. The leaf, rhizome, and stem part of the plant were extracted using hexane, ethyl acetate, and water. These extracts were then screened for their bioactivity using agar-well diffusion method and were further evaluated for their minimum inhibitory concentration (MIC). The zones of inhibition ranged between 0 - 10 mm, while its MIC ranged from >360 - 180 . tg/pL. Stem hexane and stem ethyl acetate showed good antimicrobial activity against S. aureus and S. typhimurium, however the extracts showed to be the least effective against L. monocytogenes. Compounds of the two extracts were separated using thin-layer chromatography (TLC) with a solvent system of hexane: ethyl acetate 9: 1. The TLC managed to separate a compound with an Rf value of 0.382. Further research on its active compounds should be done to justify the traditional use of the plant and the potential use of it as an antimicrobial agent. Keywords: Red jerangau, antimicrobial, well diffusion, minimum inhibitory
| Item Type: | Final Year Project Report |
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| Additional Information: | Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2018. |
| Uncontrolled Keywords: | Red jerangau, Antimicrobial, Well diffusion, Minimum inhibitory concentration |
| Subjects: | Q Science > QK Botany |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology |
| Depositing User: | Unai |
| Date Deposited: | 07 Apr 2021 08:04 |
| Last Modified: | 21 May 2024 01:43 |
| URI: | http://ir.unimas.my/id/eprint/35052 |
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