Isolation and Characterisation of ABCC1 promoter sequence Danio rerio

Anne Marian, Joseph (2018) Isolation and Characterisation of ABCC1 promoter sequence Danio rerio. [Final Year Project Report] (Unpublished)

PDF (Please get the password by email to repository@unimas.my, or call ext: 3914/ 3942/ 3933) Anne Marian ft.pdf Restricted to Registered users only Download (8MB)

Abstract

The ABCCI promoter sequence is one of the collectively mediates the ATP-dependent transmembrane efflux of multiple anticancer drug and other xenobiotics. It is also a transportation for the hydrophobic and anionic molecules such as glucuronide and glutathione (GSH) conjugation. By using isolation and cloning principle, this research is able to clone the ABCCI promoter sequence into the pZsGreen 1-1 plasmid for it to be microinjected into the Danio rerio to observe at which developmental stage of the Danio rerio is expressed. By using a pair of primers, forward 5'-TTTGGAACCACTTTAGGTTG-3' and reverse 5'- AACCACTGCTGTGTGTTATG-3' designed to isolate the promoter region of the gene sequence, where the product is able to derive 1442 bp in length. Sequencing result of 96% similarity to the Zebrafish DNA sequence from clone DKEYP-72E1 in linkage group 3 with Evalue of 0.0. In which also, fluorescence was obtained at 144 hpf at the heart of the zebrafish. These results may suggest that the promoter gene expression starts at the protruding mouth developmental stage of the fish which is beneficial to future cancer treatment studies in the later years.

Actions (For repository members only: login required)

View Item