Isolation and characterization of plant pathogenic fungi from rotten papaya

Jamie Jeremy, Jimmy (2015) Isolation and characterization of plant pathogenic fungi from rotten papaya. [Final Year Project Report] (Unpublished)

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Abstract

Plant pathogenic fungi are one of the causing agent that infecting pre-harvest and post-harvest fruits with diseases. In this study, papaya were the main sample where sample were collected and transported back to the Molecular Genetic Laboratory, Faculty of Resource Science and Technology, UNIMAS for further analysis. The sample were then cultured onto Potato Dextrose Agar (PDA) to isolate the possible pathogenic agents. Pure culture of each observed fungus was purified using sub-culturing technique then microscopic technique applied for identification of specific isolates. A total of seven fungi, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Mucor spp., Alternia spp., Coletotrichum gloeosporioides, and Penicillium spp., belonging to five genera were isolates. The most frequent isolated fimgi from the soft rot fruit was Mucor spp., while Aspergillus niger was the most frequently isolated from the stem (57.1 %) and leaf (42.9%). Colletotrichum gloeosporioides was isolated from the fuits, but not from the soft rot infected stems and leaves. The most frequently isolated fungi from the sooty rot was Penicillium spp. (56.3%) and from white fluffy rot (43.8%). Pathogenicity tests presumptively identified Mucor spp. as the most fundamental pathogen associated with soft rot of papaya fruit.

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