Production of Bio-polymer by Enterococcusfaecium no. 78 (PNCM-BIOTECH 10375)

Goh, Adrian Wai Keong (2015) Production of Bio-polymer by Enterococcusfaecium no. 78 (PNCM-BIOTECH 10375). [Final Year Project Report] (Unpublished)

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Abstract

The purpose of this project is to identify the viability of the microorganism Enterococcus faecium no. 78 (PNCM-BIOTECH 10375) of synthesize biopolymer under fermentation. It is reported that E. faecium CRL201 is capable of producing capsular polysaccharide (CPS). Therefore, E. faecium was grown under different fermentation conditions using Liquefied Sago Starch (LSS) at the concentrations of 20, 40, 60, 80 and 100 g/l. The fermentation conditions for the bacteria were fixed at a pH of 6.5, temperature of 30°C and agitation speed of 250 rpm. The results showed that E. faecium is able to produce CPS, however, it was associatted with the concentration of substrate. The exopolysaccharide (EPS) produced was around 20% of the substrate feed. It was clear that the lactic acid produced is a product apart of lactic acid. In the case of lactic acid only 31.89 g was produced from 55 g of substrated provided. It was demonstrated that E. faecium produced EPS at any concentration of substrate tested. Therefore, it could be worthy to evaluate the overall costing of LA production as well as its polymeric composition in order to have a clear panorama of the potential of this strain for industrial application.

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