The Effect of Combined Exposure of Sodium Dodecyl Sulphate and Diethyl Phthalate on Zebrafish Larvae Development.

Koay, Seow Khee (2015) The Effect of Combined Exposure of Sodium Dodecyl Sulphate and Diethyl Phthalate on Zebrafish Larvae Development. [Final Year Project Report] (Unpublished)

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Abstract

The fresh water ecosystem receives a variety of pollutants from anthropogenic activities. Among those pollutants, sodium dodecyl sulphate (SDS) and diethyl phthalate (DEP) deserve particular attention due to highly utilization as detergents bases. Therefore, it is important to evaluate the effect of combined exposure of SDS and DEP on aquatic organisms at risk. This is because of both of the chemicals have high possibility present and end up in the aquatic environment at the same time. It is still unclear whether the effect of both chemicals can be synergistic under combined exposure compare to each individual exposure. To study this research, Zebrafish (Danio rerio) was used as a test species due to it benefit on transparency of chorion, translucency of embryo, rapid embryonic development, high rate of fecundity and simple maintenance. The effect of combined exposure of SDS and DEP on Zebrafish development was determined by temporal development, which is hatching, free swim and survival rate. Besides, the larvae were also observed by phenotypic devetopjnent during the treatment period. The result of the effect of combined exposure of SDS and DEP on Zebrafish temporal larvae development and phenotypic development are synergistic compare to individual exposure.

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