Sequential Saccharification and Simultaneous Fermentation of Sugarcane Bagasse for Bioethanol Production

Ellyzeberth Linda, Linggoh (2015) Sequential Saccharification and Simultaneous Fermentation of Sugarcane Bagasse for Bioethanol Production. [Final Year Project Report] (Unpublished)

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Abstract

In this era of globalisation, fossil fuel has become the most important resources especially in the transportation industry. The realisation of the fossil fuel as the finite resources and will deplete in the future thus led to the introduction of bioethanol as the alternative to the conventional fuel. The objective of this study was to investigate the production of bioethanol from sugarcane bagasse via the process of Sequential Saccharification and Simultaneous Fermentation. Saccharomyces cerevisiae was used as the fermenting agent in this study. SSSF was conducted for 5 days and sampling was done for 0, 6, 12, 24, 30, 36, 48, 60, 72, 96 and 120 h. Total carbohydrates, reducing sugar as well as fermentation products produced were analysed. The production of ethanol was higher in 2.50% (w/v) feedstock loading compared to that in 1.25% (w/v). The highest amount of bioethanol produced was 3.73 g/L by the 2.50% (w/v) feedstock. The highest total carbohydrate produced was 4.35 g/L for 2.50% (w/v) 4.18 g/L for 1.25% (w/v). Meanwhile, the highest amount of reducing sugar produced was 0.27 g/L for 2.50% (w/v) and 0.21 g/L for 1.25% (w/v). In conclusion, Bioethanol can be produced via SSSF with the help of S. cerevisiae.

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