Phytochemical Studies and Biological Activities of Nepenthes mirabilis

Prescilla Shudden, Jungan (2014) Phytochemical Studies and Biological Activities of Nepenthes mirabilis. [Final Year Project Report] (Unpublished)

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Abstract

Nepenthes mirabilis is the tropical plant that have evolved prey capturing traps and able to utilize their prey degradation products as nutritional precursors. Extraction of Nepenthes mirabilis was carried out by using methanol. Thin Layer Chromatography (TLC), Column Chromatography and Preparative Thin Layer Chromatography (PTLC) techniques were used for the isolation and purification. Structure elucidation was determined by using Fourier Transform Infrared Spectrometer, Nuclear Magnetic Resonance and Gas Chromatography-Mass Spectrometry. Biological activity was evaluated towards brine shrimp toxicity test, antibacterial assay and anti-termites assay. Crude extract of root was found to be more toxic as compared to stem crude extract with the LC50 of 47.9 μm/ mL. All the crude extracts were toxic against the termiticidal activity with the LCso 2.6 %, 3.2 % and 4.3 % for stem, leaf and root extracts respectively. Antibacterial assay showed that all the crude extracts have the inhibition zone at 6.05 mm while for the fraction 4 of root extract showed the inhibition zone at 8 mm. For antioxidant assay, the crude extracts of Nepenthes mirabilis did not show free scavenging activity.

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