Effects of Surfactant Tween 80 on Degradation of Heterocyclic Hydrocarbon by Marine Bacteria

Li, Hie Ming (2014) Effects of Surfactant Tween 80 on Degradation of Heterocyclic Hydrocarbon by Marine Bacteria. [Final Year Project Report] (Unpublished)

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Abstract

Surfactants, also known as surface active agents, are capable to reduce the surface tension of water. Surfactants can enhance the mode of degradation and prevention of pollution in the marine environment. Biodegradation process can be enhanced by surfactants to increase the bioavailability of pollutants in marine environment. Heterocyclic hydrocarbon poses toxic activities that will lead to serious environmental problems. Bacterial strain FNSO1 was maintained on ONR7a suspension supplied with heterocyclic hydrocarbon as sole carbon source. ONR7a supplied with DBT changed to pink color while broth with DBF changed to yellow color. Toxicity test of Tween 80 at different concentration was done by plate count method and by turbidity test using UV-visible spectrophotometer. In this study, UV-visible spectrophotometer is used to investigate the component of heterocyclic hydrocarbon degradation by bacteria strain FNSO1. Different concentration of Tween 80 allowed the growth of bacteria strain FNSO1 means that Tween 80 was non toxic. In addition, non ionic surfactant, Tween 80 was tested to study its effect towards the strain FNSO 1. 0.01 % of Tween 80 with 0.1 % w/v DBT and 0.005% of Tween 80 with 0.1 % w/v DBF do not enhance the degradation but showed the highest concentration of intermediate metabolite as the accumulation of metabolite at the highest peak.

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