Characterization of the Expression Patterns of RPL37A, CTNNBI and ATBFI Genes in Normal and Tumour Human Cell Lines

Liew, Jia Lee (2014) Characterization of the Expression Patterns of RPL37A, CTNNBI and ATBFI Genes in Normal and Tumour Human Cell Lines. [Final Year Project Report] (Unpublished)

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Abstract

Ribosomes are essential component of ribosomal biogenesis and protein translation. However, it is also linked to the cancer development. Previous study shows that RPL37A is downregulated in nasopharyngeal carcinoma (NPC) cell lines compared to the normal nasopharyngeal cell line. Meanwhile, previous bioinformatics analysis revealed the interaction of Ribosomal Protein L37A (RPL37A) with beta-catenin (CTNNBI) and AT-binding transcription factor I (ATBFI). The participation and interaction of these genes in the NPC development remains uncertain due to lack of experimental evidence. In this study, comparative gene expression of RPL37A, CTNNBI and ATBF1 genes in NPC cell line compared to normal cell line is conducted by using Reverse-transcriptase PCR (RT-PCR). The down-regulation of RPL37A and ATBFI genes is observed in all of the NPC-derived cell lines when compared to normal cell line while CTNNBI is up-regulated in all of the NPC-derived cell lines. However, all of the expression shows no significant difference in the t-test (p>0.05).

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