Isolation and Characterization of Heterocyclic Hydrocarbon Degrading Bacteria from Industrial Waste Water

Doyle Amos, Suntak (2015) Isolation and Characterization of Heterocyclic Hydrocarbon Degrading Bacteria from Industrial Waste Water. [Final Year Project Report] (Unpublished)

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Abstract

Heterocyclic hydrocarbon is defined as any of a class of organic compounds whose molecules contain one or more rings of atoms with at least one atom which is the heteroatom that being an element other than carbon, most frequently oxygen, nitrogen or sulphur. There are three major heterocyclic compound used in these experiments that are carbazole, dibenzothiophene and dibenzofuran. Heterocyclic hydrocarbon are among the most prevalent and harmful environmental pollutants which can be found in the polluted sites. Heterocyclic hydrocarbons are toxic, mutagenic and carcinogenic The objectives of these study are to isolate the heterocyclic hydrocarbon such as carbazole, dibenzofiiran and dibenzothiophene degrading bacteria by using enrichment growth media with minimal salt medium (MSM) and to determine the characteristics of these compounds by using biochemical test such as gram staining, oxidase test, catalase test, methyl red test, motility test, indole test, voges-proskauer test and citrate test. The bacteria were design as P251 which can degrade DBT, P252 which can degrade DBF and P253 which can degrade CAR in the enrichment medium of MSM broth. All the unknown bacteria are expected to be in Pseudomonas sp. according to the results of biochemical tests.

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