Halal Authentication of raw meats using PCR Amplification of mitochondrial DNA

Nur Hanisah, Tun Ibrahim (2014) Halal Authentication of raw meats using PCR Amplification of mitochondrial DNA. [Final Year Project Report] (Unpublished)

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Abstract

Meat adulteration is the main concern for the consumers nowadays. Religion has fundamental factors in taboo food and special regulation in meat. Raw meats of chicken, beef, lamb and pig were examined using PCR amplification of mitochondrial DNA. Three oligoprimers were used to amplified mitochondria DNA (mtDNA) of cytochrome b (two types of primers) and mitochondrial 12s rRNA (mt-12s rDNA) (one type of primer) gene for vertebrate-specific. No band was observed for cyt b primer. Amplification product using mt-12S rDNA primer produces single band at 456 bp. While amplification product of species-specific mt cyt b primer successfully produce single band for pig DNA. At the end of this study, PCR amplification of mitochondrial DNA of cytochrome b is proven to rapid tool for pig DNA detection.

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