OPTIMIZATION OF IMMOBILIZATION MEDIA OF THALASSOSPIRA PROFUNDIMARIS : DIFFUSION AND STRENGTH STUDIES

MUHD NURUL AZAM, MUHD DRUS (2018) OPTIMIZATION OF IMMOBILIZATION MEDIA OF THALASSOSPIRA PROFUNDIMARIS : DIFFUSION AND STRENGTH STUDIES. [Final Year Project Report] (Unpublished)

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Abstract

Many petroleum samples have abundant heterocyclic compounds. One example of petroleum samples is diesel where nitrogen, sulphur and aromatic compounds are the major impurities present in it. Heterocyclic compounds are inextricably into life processes in which a vast number of active heterocyclic compounds are being used. The problem to the industry is that the waste are discharged into the sea that could treat both marine life as well as human as consumer. Bacteria called Thalassospira Profundimaris could potentially degrade the carcinogenic compound of petroleum discharge. However, having the bacteria to work without protection could shorten the lifespan of the bacteria itself where free cell would offer a multitude of disadvantages such as substrate inhibition when using free cell suspension in wastewater. It also offers low stability, lower degradability of the cell in the presence of high concentration of pollutants from the systems. Cell immobilisation offers high mechanical strength, high metabolic activity, and resistance to toxic chemicals causing damage to the cell. The diffusion barriers within the biofilm against toxic compound could save the cell from damage as well as maintaining the microbial population in carriers without any loss. The cell immobilisation is used widely in wastewater industries whereby the media used widely are gellan gum, Ca-alginate, yeast and others. In this project, the focus is on the finding of the optimum strength and diffusivity of the media itself to increase the performance of the bacteria entrapped inside as well as withstanding the harsh environment around it. With various concentration of gellan gum and Ca-alginate, this project has proven that the concentration affects the porosity and the strength of the media. The project has also proven that increasing the concentration of media would form stronger media with lower diffusivity. Lower concentration forms soft media with higher diffusivity.

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