Extraction and characterization of ligninolytic enzymes produced by tropical white rot fungus during produced by tropical white rot fungus during biopulping of acasia mangium

Faisalina, Ahmad Fisol (2005) Extraction and characterization of ligninolytic enzymes produced by tropical white rot fungus during produced by tropical white rot fungus during biopulping of acasia mangium. [Final Year Project Report] (Unpublished)

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Abstract

Pycnoponis coccineus and Coriolus versicolor are tropical white-rot basidiomycetes that degrade lignin selectively and often used in biopulping. Development of appropriate experimental procedures for recovery and determination of enzymes produced by Pycnoporus coccineus and Coriolus versicolor during biopulping are necessary to evaluate the role of each enzyme in this produced during biopulping were extracted, purified and determined it protein and lignin content. Laccase activity showed the highest rate than other lign inolytic enzymes activities which was 16.950 I.U/ml (Pycnoporus coccineus) but not in the case of Coriolus versicolor enzyme extract (2.974 I.U/ml). Manganese peroxidase activities pattern correlate with lignin peroxidase activities for both fungi. However cellulase activities were very low in both fungi enzymes extract. The enzyme activities were expressed as units per milliliter, where I U was defined as I milirnol of substrate oxidized per min. Purification of these enzymes involved hydrophobic interaction chromatography (H IC) method. HIC managed to purify the protein up to 90 and 95% purity. The specific activity of laccase (0.045 LU/ml, 0.016 LU/ml), manganese peroxidase (0.043 I.U/ml, 0.073 LU/ml) and lignin peroxidase (0.011 LU/ml, 0.022 LU/ml) of Coriolus versicolor and Pycnoporus coccineus were determined. SDS-PAGE was used to determine the purity and molecular weight of ligninolytic enzymes. The lignin content showed considerable decreased after biotreated with both fungi.

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