An attempt to detect infectious bronchitis virus in village chickens in Kuching division, Sarawak

Tan, Sang Nee (2007) An attempt to detect infectious bronchitis virus in village chickens in Kuching division, Sarawak. [Final Year Project Report] (Unpublished)

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Abstract

Most of the established detection methods of infectious bronditis virus (!BY) rely on the IBV isolation after propagation in few days old specific-pathogen-free (SPF) embryonated eggs for RNA preparation. This culture method makes the IBY detection methods become relatively mere time-consuming. Therefore, the development of IBY detection method merely involving molecular technique which has higher efficiency is becoming a major concern. In order to obtain chicken tissue samples for I BY detection, dissections are required which may lead to more time-consuming and labor-intensive laboratory work compared to blood samples collection. In this study, an attempt was made to develop a RNA extraction protocol for chicken blood sample in order to prepare RNA sample with high yield and quality to be used in Reverse- Tran;ription Polymerase Chain Reaction (RT-PCR). An attempt to detect IBY in village chickens in Kuching Division using RT-PCR was also made. Before that, an attempt was made to extract viral RNA from vaccine strain to be used as positive control for RT-PCR. However, extraction of RNA with high yield and good quality from chicken blood sample was unsuccessful. The results suggested that kidney sample was the most suitable source to obtain RNA sample from chicken compared to blood, lung and trachea samples. The IBV detection in village chickens in Kuching Division had not been accomplished due to time constraint and inadequate supply of materials for RT-PCR. Since IB can cause financial losses in poultry industry, further research on IBV detection method with high efficiency should be conducted to limit the extent of the disease during its outbreaks. The IBV detection in village chickens in Kuching Division should also be conducted due to the scarcity of the reports on prevalence of I BY in village chicken in Kuching Division.

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