Phytochemical studies of Cassia alata

Nurzahrul Rasyidah, Mohd Amin (2015) Phytochemical studies of Cassia alata. [Final Year Project Report] (Unpublished)

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Abstract

Phytochemical studies have been carried out on the Cassia alata, from the Caesalpinaceae family that commonly known as Solok, Gelenggang, Ketepeng Badak in Malaysia. Most of the recent phytochemical studies carried out in this family stated that it concentrated with sterol compounds. The methanol crude extract obtained from leaves sample was 68.15g, stems sample was 27.50 g and root sample was 9.68 g. Isolation and purification of the pure compounds were characterized by Thin Layer Chromatography (TLC) and Column Chromatography method. The isolated pure compounds were ergosterol and ß-sitosterol. The molecular structures of the pure compounds were elucidated by spectroscopic analysis using 'H and 13C Nuclear Magnetic Resonance Spectroscopy (NMR) and Fourier Transform Infrared Spectroscopy (FTIR). For biological evaluation, the methanol crude extracts of leaves, stems and roots of Cassia alata and pure compounds were tested with the brine shrimp (Artemia salina) lethality assay, antioxidant test and anti-bacterial test. The results stated that ß-sitosterol has highest LC50 value that was 1016.0 ppm and highest percentage of antioxidant activity was 72.43%. The value indicated that ß-sitosterol has less toxic against Artemia salina and high antioxidant properties. Leaves crude extracts tested on Staphylococcus aureus and Escherichia coli show the highest diameter of zone inhibition with the value of 7.7 mm and 10.0 mm. These values indicate that it has anti-bacterial properties.

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