Detection of Campylobacter spp. in Silvered Leaf Monkey (Trachypithecus cristatus)

Ag Mohd Hasbi, Ag Tuah (2015) Detection of Campylobacter spp. in Silvered Leaf Monkey (Trachypithecus cristatus). [Final Year Project Report] (Unpublished)

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Abstract

Campylobacter jejuni is one of the common causes of enteritis worldwide that can range from mild, watery diarrhoea to a dysenteric-like disease with blood. Some strains are capable of causing a rare infection known as Guillain-Barre Syndrome (GBS). C. jejuni is slender, curved and spiral rod-shaped bacteria that can be found in the intestinal tract of most mammals and birds. There are several other species under the genus Campylobacter, but the most common species to be isolated in diarrhoea sample are C. jejuni and C. coll. This study was done to determine if these bacteria found in the faecal samples of Silvered leaf monkey, Trachypithecus cristatus between two sampling sites, Bako National Park, Kuching Sarawak and Labuk Bay Proboscis monkey sanctuary, Sandakan, Sabah. The protocol used was based on ISO 10272-1: 2006, using Bolton enrichment and cultured on modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA). The identification and confirmation were done by using the primer targeting Hip-O gene in PCR. The prevalence study of Campylobacter spp. that grew on mCCDA shows that Labuk Bay Proboscis Monkey Sanctuary has higher occurrence with 68.75% compared to Bako National Park with 0%. Even though the specific species of C. jejuni cannot be identified by PCR approach, the presences of the colony on mCCDA show that the presence of Campylobacter spp. indicated that these bacteria had presence at Labuk Bay Proboscis Monkey Sanctuary.

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