Phylogenetic Relationship of Genus Leptobrachium (Amphibia: Anura: Megophridae)

Norhasimah, Jakariah (2014) Phylogenetic Relationship of Genus Leptobrachium (Amphibia: Anura: Megophridae). [Final Year Project Report] (Unpublished)

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Abstract

Genus Leptobrachium is one of genera within family Megophridae found in Sarawak. It consists of cryptic species that are difficult to distinguish. Thus, phylogenetic relationship of genus Leptobrachium was studied by using mitochondrial ribosomal of 16S rRNA. Tissue sample were collected in four localities within Sarawak namely Kubah National Park, Matang Wildlife Centre, Mt. Gading National Park, and Mt. Santubong, Sarawak by using forest transects method and quadrats method. Tissues were extracted using molecular technique approach such as DNA extraction, PCR amplification, and sequencing. Phylogenetic relationships were inferred using primer pair of mitochondrial ribosomal 16S rRNA gene. A total of 1464bp of 16S rRNA from 10 samples were analyzed using Neighbor-Joining (NJ), Maximum Parsimony (MP) and Maximum Likelihood (ML) method. For this study, monophyletic group were formed consist of all species within the genus Leptobrachium with respect to outgroup. It is proven that 16S rRNA gene was useful as gentic marker to elucidate phylogenetic study within genus level. The study of phylogenetic relationship of genus Leptobrachium able to infer the divergence and lineage of species within respected genus thus contribute to provide future reference at local level.

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