Isolation and characterisation of antimicrobial producing bacteria from soil against phytophthora spp

Lembat, Delstein Leman (2013) Isolation and characterisation of antimicrobial producing bacteria from soil against phytophthora spp. [Final Year Project Report] (Unpublished)

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Abstract

Piper nigrum L. is one of the important spices and the need of the piper nigrum L. is increasing. It is widely used in medicinal, aromatherapy and cuisine. However, the infection by the Phytophthora species is leading to the decrease of the Piper nigrum L. quality. In order to reduce the infection, chemicals, such as Bordeaux mixture and copper oxychloride are widely used. However, the chemicals not only can pollute the environments, but it is also can kill the non-target species. Therefore, the aim of the study is to isolate bacteria that can produce antimicrobial properties against the Phytophthora capsici. In this study, soil samples were collected from the Piper nigrum L.’s farm and the bacteria that inhibit the Phytophthora capsici was undergo secondary screening via agar-well diffusion method. Then, five selected bacteria were characterized by using Polymerase Chain Reaction (PCR) and DNA sequencing. Thus, the bacteria that were able to inhibit the Phytophthora capsici are Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas syringae and Pseudomonas brassicacearum.

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