Isolation and characterization of amylolignocellulolytic fungi from sago industrial waste

Siti Nur Hidayah, Bujang (2011) Isolation and characterization of amylolignocellulolytic fungi from sago industrial waste. [Final Year Project Report] (Unpublished)

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Abstract

Total five fungi isolates were found from sago industrial waste. Screening of the fungi isolates was done by starch agar plate, RBBR dye decolourization and CMC-Congo Red method. One of the isolate was negative for amylase activity and all four isolates were positive for cellulase activity. Only two isolates were positive for ligninase activity and overall screening results showed only two isolates were positive for all three enzymes. Molecular characterization was done and PCR product size between 600 - 750bp was obtained when amplified with ITS primers. Isolate that was positive for amylolignocellulolytic identified as Tinctoporellus sp. Solid State Fermentation (SSF) using sago waste as a substrate was done to analyze enzyme activity. Enzyme activity for amylase, cellulase, lignin peroxidase, manganese peroxidase and laccase were 2.457 umol/min, 19.575 umol/min, 13.352 umol/min, 2.955 umol/min and 23.233 umol/min respectively.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2011.
Uncontrolled Keywords: Amylolignocellulolytic fungi, solid state fermentation, sago waste, FSTS, 2011, Universiti Malaysia Sarawak, UNIMAS, universiti, university, Borneo, Malaysia, Sarawak, Kuching, Samarahan, IPTA, education, undergraduate, research
Subjects: Q Science > Q Science (General)
S Agriculture > S Agriculture (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 05 Dec 2013 08:04
Last Modified: 09 Feb 2024 02:22
URI: http://ir.unimas.my/id/eprint/324

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