Isolation of Escherichin coli Specific Phages from Sewage Water

Yee, Xavier Kar Seng (2017) Isolation of Escherichin coli Specific Phages from Sewage Water. [Final Year Project Report] (Unpublished)

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Abstract

Escherichia coli is one of the most intensively studied and best understood bacteria due to its hardiness, versatility and ease of handling. The versatility of this species of bacteria allows it to inhabit in diverse ecological niches including soil and waler sources, and also the human gut. However, the rising of antibiotic resistance in pathogenic E. coli is a serious threat to human health. One potential alternative to antibiotics is phage therapy. By isolating and studying the stability of E.coli specific phages (coliphages), we are able to determine the effectiveness of the coliphages against different strains of E. coli. In this study, a number of different coliphage strains had been isolated from the environment. Futhermore, a certain strain of the isolated colihage shows great lysing capability against a number of clinical E. coli strains. Further characterization of the coliphage through heat treatments indicates that the stability of the coliphage varies greatly depending on the different strain type. Besides that, exposure to various pH indicates the phages are unable to survive in extreme pH conditions.

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