Toxicogenomic Screening on Dipropyl Phthalate Treated Embryos

Teh, Kai Jia (2017) Toxicogenomic Screening on Dipropyl Phthalate Treated Embryos. [Final Year Project Report] (Unpublished)

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Abstract

Dipropyl phthalate (DPrP), belongs to the phthalate ester (PAEs) family and is widely used as addictive in the plastic products. could be released into the environment through commercial products and food. and it might cause impact to human health by several pathways. Several PAEs had been reported could act as estrogen and thyroid endocrine disruptor. The aim of this research is to test the toxic effect of DPrP by using Zebrafish (Danio rerio) embryo as the animal model. Zebra fish eggs were treated with nine different DPrP concentrations from 4 hour post-fertilization (hpf) until 96 hpf. DPrP had induced the lethal effect in the embryos. ,vith LC() was calculated as 5.16 mg/L. Phenotypic analysis using Zebrafish embryo has revealed that DPrP can cause uninf1ated swimbladder, pericardia! oedema. yolk sac oedema. shorten body length. yolk opaque. microphthalmia in Zebrafish embryos, and the hatching ability had been affected too. Using toxicogenomic screening. gene expression of tr that involved in thyroid hormone metabolism were examined by using semiquantitative Reverse-Transcript PCR. Result shown that the band intensity of iur gene was altered and the effect was dose-dependent. Overall. these results demonstrate that DPrP can cause morphology deformations in Zebrafish embryo and it might be associated with the effect of thyroid endocrine disruptor.

Item Type:	Final Year Project Report
Additional Information:	Project report (B.Sc.) -- Universiti Malaysia Sarawak, 2017.
Uncontrolled Keywords:	Phthalate ester, Dipropyl phthalate, Toxicogenomic, ttr, Zebrafish, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects:	Q Science > QR Microbiology
Divisions:	Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User:	Patrick
Date Deposited:	19 Oct 2020 03:41
Last Modified:	26 Mar 2024 02:44
URI:	http://ir.unimas.my/id/eprint/32215

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