Cloning of green fluorescence protein gene into binary vector

Francis, Ruth Grace (2013) Cloning of green fluorescence protein gene into binary vector. [Final Year Project Report] (Unpublished)

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Abstract

Green Fluorescent Protein (GFP) gene derived from the bioluminescent jellyfish, Aequoera victoria has been widely used in the field of molecular biology where it is utilized as reporter gene of many transformation events. The simplicity of this reporter gene has made it as one of the most stable reporter gene in plant transformation. Through times, a lot of researches have been done to study the applications of gfp gene in plants. In this project, a modification has been done on the genetic material of the binary vector, pGSA1131 which involved the removal of the destructive gus gene via restriction enzyme digestion followed by the blunting of gfp gene and vector ends, ligation, transformation of ligated vector and finally analysis of transformants. Based on the analysis, it is said that the cloning of the gfp gene obtained from plasmid MCB30 into binary vector pGSA1131 was not successful.

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