Bioconversion of Local Agro-Waste into Protein Enriched Substrate using Aspergillus versicolor

Suhana, Sanusi (2017) Bioconversion of Local Agro-Waste into Protein Enriched Substrate using Aspergillus versicolor. [Final Year Project Report] (Unpublished)

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Abstract

The use of microorganism specifically, Aspergillus spp. in the bioconversion of local agrowastes show high potential in the production of protein enriched substrates which can be further used as animal feed. The potential of solid state fermentation can be seen in minimising the accumulation of local agro-wastes and their negative impacts towards the environment. In this study, 5 g ofbanana peel, pineapple peel and papaya peel with the initial moisture content of70% were used as the SSF substrates. Three plugs ofculturedAspergillus versicolor were incubated with each substrates in separate conical flask for 6 days at 30 DC. After fermentation process was completed, protein sample was extracted and the protein content was analysed using Bradford method. Pineapple peel was identified as the best local agro-waste for production of protein-enriched substrate where the protein produced was 0.345 mg/mt. SSF parameters was optimised using pineapple peel as the substrate in which 0.345 mg/ml of protein was produced when 70% ofinitial moisture content and incubation temperature of 30 DC were used. An amount of 0.417 mg/ml was produced when SSF was performed for 8 days.

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