The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses

Yeo, Freddy Kuok San and Ting, Hieng Ming and Cheah, Boon and Chen, Yu Cheng and Yeh, Pei-Min and Cheng, Chiu Ping and Vie, Ane Kjersti and Rohloff, Jen and Winge, Per and Bones, Atle M. and Kissen, Ralph (2020) The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses. Frontiers in Plant Science, 11 (257). pp. 1-18. ISSN 1664-462X

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Abstract

Glucosinolates are defense-related secondary metabolites found in Brassicaceae. When Brassicaceae come under attack, glucosinolates are hydrolyzed into different forms of glucosinolate hydrolysis products (GHPs). Among the GHPs, isothiocyanates are the most comprehensively characterized defensive compounds, whereas the functional study of nitriles, another group of GHP, is still limited. Therefore, this study investigates whether 3-butenenitrile (3BN), a nitrile, can trigger the signaling pathways involved in the regulation of defense responses in Arabidopsis thaliana against biotic stresses. Briefly, the methodology is divided into three stages, (i) evaluate the physiological and biochemical effects of exogenous 3BN treatment on Arabidopsis, (ii) determine the metabolites involved in 3BN-mediated defense responses in Arabidopsis, and (iii) assess whether a 3BN treatment can enhance the disease tolerance of Arabidopsis against necrotrophic pathogens. As a result, a 2.5 mM 3BN treatment caused lesion formation in Arabidopsis Columbia (Col-0) plants, a process found to be modulated by nitric oxide (NO). Metabolite profiling revealed an increased production of soluble sugars, Krebs cycle associated carboxylic acids and amino acids in Arabidopsis upon a 2.5 mM 3BN treatment, presumably via NO action. Primary metabolites such as sugars and amino acids are known to be crucial components in modulating plant defense responses

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