Skin structure difference in tree-frogs (Genus Polypedates) at Kubah National Park, Sarawak, Borneo

Shahrul Rizza, Razali (2017) Skin structure difference in tree-frogs (Genus Polypedates) at Kubah National Park, Sarawak, Borneo. [Final Year Project Report] (Unpublished)

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Abstract

Frog's pond is a natural breeding ground for borneon frogs especially the tree-frogs from the Genus Polypedates consisting of four species; Polypedates colletti, Polypedates othilopus, Polypedates macrotis and Polypedates leucomystax. Their ability to adapt well in xeric environment shows the important of the genus indirectly as an indicator to the environment above the leaf litters. Histological techniques and procedure is being used to obtain the skin sample. Hematoxylin and Eosin staining technique is used to stain four region of skin sample; Dorsal Head(DH), Dorsal Centre(DC), Ventral Head(VH) and Ventral Centre(VC). One-way ANOVA is used in differentiating between the four species of three frogs with parameters based on the glands numeration, distance between glands and area ratio of glands. SPSS software is used to analyse the data being extracted. In this genus, seromucous glands has the highest numeration compared to mucous and granular glands. P. othilopus shows of having large and conspicuous granular glands that might contributed to their unpleasant smell from their moist skin. Future studies and research should take account on the gender and maturity of frogs in the analysis.

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