The effect of mouthwash applications on oral microiome by bacterial detection using 16S RRNA in Saliva Sample

Nur Shafiqah Hanim, Rakwi (2017) The effect of mouthwash applications on oral microiome by bacterial detection using 16S RRNA in Saliva Sample. [Final Year Project Report] (Unpublished)

PDF (Please get the password by email to repository@unimas.my , or call ext: 082-583914/3973/3933) Nur Shafiqah Hanim.pdf Restricted to Registered users only Download (4MB)

Abstract

This research is focused on investigating the effectiveness of mouthwash by analyzing the DNA yield and quality, and to detect the presence of bacteria in DNA extracted from saliva via 16S rRNA PCR. The DNA was extracted from the saliva sample of with and without using mouthwash by an individual using Phenol- Chloroform extraction method. The DNA extract then evaluated using Nano Drop ND-1000 UVNIS Spectrophotometer used to determine the DNA quality and DNA yielding. After that, the 16S rRNA gene was amplify for bacterial detection in the saliva using 27 F and 1492R primers, the PCR product then observes on the gel electrophoresis. As the result of this study, the sample from individual F using mouthwash has higher DNA yielding (36.3 ng/μL) compare to the sample without using mouthwash (21.6 ng/gL). However, the contamination was detected in low ratio of absorbance at A26o/A280an d A260/A23oT. he amplification of 16S rRNA gene also successful and can be observe on the band detected at the gel electrophoresis, marked at 1500 bp. marker. To conclude, the mouthwash are prove to be effective in reducing the number of oral microbial, detected by the ratio absorbance, A2801260a nd A2301260T. he amplification of the 16S rRNA gene are unable to prove the effectiveness and further gene sequencing is required.

Actions (For repository members only: login required)

View Item